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- W2360808531 abstract "Cellular glutathione peroxidase(cGPX) is an important antioxidase in animals. This experiment setsup a method that can beused for quantitative analysis cGPX mRNA in RT PCR process. β Actin is used forinternal standard. Total RNA was extracted from livertissue of broiler. The mRNA was reverse transcripted and the cDNA fragmentsof cGPX gene and β Actin gene were amplified by RT PCR using the gene specific primers.The integrated optical density(IOD) was showed by scanning the electrophoresis bands of PCR products with VDS system. By studying the range of PCR products linearity amplification, the best of all cycle number and the volume of template(cDNA) were made certain. The relative concentration of cGPX mRNA can be calculated by the IOD ratio of cGPX and β Actin." @default.
- W2360808531 created "2016-06-24" @default.
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- W2360808531 date "2003-01-01" @default.
- W2360808531 modified "2023-09-22" @default.
- W2360808531 title "Quantitative analysis for cellular glutathione peroxidase mRNA in broiler hepar" @default.
- W2360808531 hasPublicationYear "2003" @default.
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