Matches in SemOpenAlex for { <https://semopenalex.org/work/W2360840493> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2360840493 abstract "Objectives To obtain the non-fusion expression vector pIRES-p53-p14~(ARF) and de- termine its effect on MG-63 cells proliferation.Methods p53 and p14~(ARF) full-length cDNA coding region were subcloned into the internal ribosome entry site (IRES) vector one by one by using gene recombination technology.The constructed plasmids were identified by polymerase chain reaction (PCR) in combination with restriction enzyme digestion and sequence analysis.The recombinat plasmids pIRES-p53-p14~(ARF) was transfected into MG-63 cells by lipofectamin 2000.After 48 hours, its expression in cells was observed by inverted microscopy (Axiovent 2000) and immunohistochem- istry,and its tumor-inhibiting effects were preliminarily examined.Results The recombinant plas- mid pIRES-p53-p14~(ARF) was successfully constructed.The recombinant plasmid was in consistency with the experimental design as confirmed by RT-PCR and restriction enzyme digestion.In the ge- home of these transfected target cells,the expression of p53 and p14~(ARF) and protein were immunohis- tochemically detected in vitro.After the transfection,sporadic apoptosis could be seen in the tumor cells.Conclusions The non-fusion expression vector pIRES-p53-p14~(ARF) was successfully construc- ted and expressed." @default.
- W2360840493 created "2016-06-24" @default.
- W2360840493 creator A5064899978 @default.
- W2360840493 date "2007-01-01" @default.
- W2360840493 modified "2023-09-23" @default.
- W2360840493 title "Construction of Non-fusion Expression Vector of Single Promoter for p53/p14~(ARF) and Assessment of Its Expression in MG-63 Cells Proliferation" @default.
- W2360840493 hasPublicationYear "2007" @default.
- W2360840493 type Work @default.
- W2360840493 sameAs 2360840493 @default.
- W2360840493 citedByCount "0" @default.
- W2360840493 crossrefType "journal-article" @default.
- W2360840493 hasAuthorship W2360840493A5064899978 @default.
- W2360840493 hasConcept C104317684 @default.
- W2360840493 hasConcept C105580179 @default.
- W2360840493 hasConcept C111829193 @default.
- W2360840493 hasConcept C128319531 @default.
- W2360840493 hasConcept C149364088 @default.
- W2360840493 hasConcept C153911025 @default.
- W2360840493 hasConcept C187882448 @default.
- W2360840493 hasConcept C22744801 @default.
- W2360840493 hasConcept C33161422 @default.
- W2360840493 hasConcept C40767141 @default.
- W2360840493 hasConcept C54009773 @default.
- W2360840493 hasConcept C54355233 @default.
- W2360840493 hasConcept C77430603 @default.
- W2360840493 hasConcept C86803240 @default.
- W2360840493 hasConcept C91779695 @default.
- W2360840493 hasConceptScore W2360840493C104317684 @default.
- W2360840493 hasConceptScore W2360840493C105580179 @default.
- W2360840493 hasConceptScore W2360840493C111829193 @default.
- W2360840493 hasConceptScore W2360840493C128319531 @default.
- W2360840493 hasConceptScore W2360840493C149364088 @default.
- W2360840493 hasConceptScore W2360840493C153911025 @default.
- W2360840493 hasConceptScore W2360840493C187882448 @default.
- W2360840493 hasConceptScore W2360840493C22744801 @default.
- W2360840493 hasConceptScore W2360840493C33161422 @default.
- W2360840493 hasConceptScore W2360840493C40767141 @default.
- W2360840493 hasConceptScore W2360840493C54009773 @default.
- W2360840493 hasConceptScore W2360840493C54355233 @default.
- W2360840493 hasConceptScore W2360840493C77430603 @default.
- W2360840493 hasConceptScore W2360840493C86803240 @default.
- W2360840493 hasConceptScore W2360840493C91779695 @default.
- W2360840493 hasLocation W23608404931 @default.
- W2360840493 hasOpenAccess W2360840493 @default.
- W2360840493 hasPrimaryLocation W23608404931 @default.
- W2360840493 hasRelatedWork W2347497859 @default.
- W2360840493 hasRelatedWork W2349384855 @default.
- W2360840493 hasRelatedWork W2352176312 @default.
- W2360840493 hasRelatedWork W2355728310 @default.
- W2360840493 hasRelatedWork W2357504759 @default.
- W2360840493 hasRelatedWork W2361240596 @default.
- W2360840493 hasRelatedWork W2363191578 @default.
- W2360840493 hasRelatedWork W2367671132 @default.
- W2360840493 hasRelatedWork W2372952044 @default.
- W2360840493 hasRelatedWork W2376626603 @default.
- W2360840493 hasRelatedWork W2378091501 @default.
- W2360840493 hasRelatedWork W2385601593 @default.
- W2360840493 hasRelatedWork W2386033801 @default.
- W2360840493 hasRelatedWork W2386061464 @default.
- W2360840493 hasRelatedWork W2393375784 @default.
- W2360840493 hasRelatedWork W2411724880 @default.
- W2360840493 hasRelatedWork W2467801310 @default.
- W2360840493 hasRelatedWork W3002284046 @default.
- W2360840493 hasRelatedWork W3031280610 @default.
- W2360840493 hasRelatedWork W3030751603 @default.
- W2360840493 isParatext "false" @default.
- W2360840493 isRetracted "false" @default.
- W2360840493 magId "2360840493" @default.
- W2360840493 workType "article" @default.