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- W2360846688 abstract "[Objective] To investigate the effects on breast cancer cell line by stable transfection and analysis the cytobiology of the cells transfected by pcDNA3.1 vector. [Methods] Eukaryotic expression vector, pcDNA3.1, was transfected into human breast cancer cell line T47D by Effectene. Stable transfectants were selected by G418. Cytomorphological analysis, cell proliferation, the cloning efficiency in soft agar and the migration assay in vitro were used to evolution the cytobiology of transfected cells. [Results] Comparing with the parental cell line and other cells transfected with same vector, pcDNA-4 had evident change in cytomorphology, increasing ability to form cell colonies in soft agar, and ability of cell migration. [Conclusion] The cytomorphological and cytobiological changes of breast cancer cell can be induced by transfection of the blank eukaryotic expression vector." @default.
- W2360846688 created "2016-06-24" @default.
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- W2360846688 date "2006-01-01" @default.
- W2360846688 modified "2023-09-25" @default.
- W2360846688 title "Effect of stable transfection with eukaryotic expression vector on breast cancer cells growth" @default.
- W2360846688 hasPublicationYear "2006" @default.
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