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- W2360847873 abstract "Objective To observe the drug resistance and tumorigenic ability of side population cells(SP cells) which were sorted from BGC-823 cell lines.Methods SP cells were collected from BGC-823 cells by flow cytometry(FCM).The cell proliferation of SP cells and non-SP cells were measured by methabenzthiazuron(MTT) assay.The FCM was employed to investigate the inhibitory effect of different concentrations of 5-fluorouracil(5-FU) on BGC-823 cells and measure the proportion and cell cycle of SP cells.The tumorigenic ability of the SP cells was assessed by in vivo transplantation into non-obese diabetic/severe combined immuno-deficiency(NOD/SCID) mice.Results There was 0.9%-2.1% SP cells in BGC-823 cells.It showed that SP cells had a higher proliferative ativity than non-SP cells by MTT assay.The cell numbers of BGC-823 treated by 2.5,5,10,25μg/ml 5-FU for 36h were 2.6×106,2.3×106,2.0×106,1.8×106;and the proportions of SP cells were 12.9%,9.9%,4.0%,2.1%,respectively.At the same time,it demonstrated that sorted SP cells has the tougher viability than non-SP cells when suffering with drug treatment.It showed that SP cells were in G0/G1 phase by FCM.The average weights of tumors formed by SP cells(1×104) and non-SP cells(1×104) were 0.577g and 0.150g,respectively.SP cells possessed higher tumorigenicity than non-SP cells,such as tumor growth time and tumor weight.Conclusion SP cells from human gastric cancer cell line BGC-823 possessed a higher proliferative activity and stem cell-like drug-resistence character.The research of SP cells could provide new targets for anti-tumor therapy." @default.
- W2360847873 created "2016-06-24" @default.
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- W2360847873 date "2012-01-01" @default.
- W2360847873 modified "2023-09-23" @default.
- W2360847873 title "A preliminary study on drug resistance and tumorigenic ability of side population cells in gastric cancer" @default.
- W2360847873 hasPublicationYear "2012" @default.
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