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- W2360850146 abstract "Randomly amplified polymorphic DNA (RAPD) was used to study Grateloupia filicina and Sinotubimorpha porracea (Rhodophyta), in terms of the molecular systematics. Amplification with 60 random primers was made under predetermined optimal reaction (samples were first heated at 95℃ for 10 min., and followed by 45 cycles of 60 sec. at 94℃, 100 sec. at 36℃, and 160 sec. at 72℃, then held at 72℃ for 10min. after 45 cycles). 21 of the 60 random primers tested were positive, generating a total of 120 prominent amplification products. The amplified fragments were scored as present (1) of absent (0) for each DNA sample and an index of degree of band sharing ( S ) was calculated by using Nei and Li's matching coefficient method. The value of (1- S ) was used to evaluate genetic distances between species. The dendrograms were built based on UPGMA and Neighbor-Joining analysis of genetic distance matrix data. The result suggests that RAPD is useful in distinguishing G.filicina from S. porracea and that G.filicina and S.porracea may belong to different genera." @default.
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- W2360850146 date "2000-01-01" @default.
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- W2360850146 title "RAPD analysis of Grateloupia filicina and Sinoyubimorpha porracea" @default.
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