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• W2360879284 abstract "Background Overproliferation of keratocyte is the protagonist of corneal wound healing and scar formation.Cyclin-CDK-CKI system is the endogenous modulating system of cell proliferation.Among the system,cyclinD1 gene is the key factor and provide targets for gene therapy.ObjectiveThis experiment was to investigate the influence of transfer of cyclinD1 antisense gene on the proliferation of human keratocyte in vitro.MethodsHuman cornea was obtained from the Eye Bank of this hospital.Human keratocytes were cultured in vitro according to our previous method,and the second and third generation of cells were used in this study.Cationic liposome-mediated cyclinD1 antisense gene was transfected into cultured human keratocytes as cyclinD1 transfection group,and only lipofectamine was transfected in lipofectamine transfection group.In non-transduction group,PBS was used instead of transfection of cyclinD1.The expression of cyclinD1 protein in the human keratocytes was identified by immunohistochemistry at 2,6,10 days after gene transduction.The proliferation of cultured keratocytes in different groups were detected by MTT.Cellular viability was detected using 0.5% trypan blue.ResultsLittle amount of positive keratocyte nucleus were seen in cyclinD1 transduction group in comparison with non-transduction group and lipofectamine-transduction group.The expressions of cyclinD1 protein(A values)in human keratocytes were significantly decreased in cyclinD1 transduction group during 2,6,10 days after transduction in comparison with non-transduction group(t= 6.050,t=5.180,t=5.040,P0.01)and lipofectamine transduction group(t=6.190,t=5.670,t=5.010,P0.01).The A570 values of cultured keratocytes was considerably lower in cyclinD1 transduction group compared with non-transduction group(t=5.830,t=5.210,t=4.920,P0.01)and lipofectamine transduction group(t=5.130,t=5.010,t=4.850,P0.01)in 2,6,10 days after transduction.No obviously differences were found in the expression of cyclinD1 protein and the proliferation of human keratocyte between non-transduction group and lipofectamine transduction group through the experimental duration(P0.05).Trypan blue dye revealed that the rates of cellular viability in all groups were more than 90%.ConclusionCyclinD1 antisense gene transduction mediated by cationic liposome into cultured human keratocytes can inhibit the proliferation of human keratocyte.The study provide a preliminary theoretical basis for the application of cyclinD1 antisense gene transduction in controlling corneal wound healing and scar formation." @default.
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• W2360879284 date "2010-01-01" @default.
• W2360879284 modified "2023-09-26" @default.
• W2360879284 title "Inhibition of human keratocyte proliferation following the transfection of cyclinD1 antisense gene" @default.
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