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- W2360949191 abstract "Objective To build a simple and fast method for salmonella detection by the technology of electrochemical DNA biosensor.Methods New distilled pyrrole and multiwalled nanotubes were polymerized onto the surface of carbon glass electrode by cyclic voltammetry to form a robust and well-distribute film,which contained carboxylic groups.Conductive polymer film combines the advantages of the fine conductivity of carbon nanotubes and the large active surface area of polypyrrole.Avidin and carboxyl on the electrode surface were crosslinked by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride(EDAC).Then,biotin-ssDNA was immobilized on the modified electrode surface via biotin-avidin system.Results The technique of A.C.impedance was applied to detect impedance(Ret) signal of target DNA.The range of linearity was 1.0×10-9mol/L~1.0×10-7mol/L.The detection limit was 5.0×10-10mol/L.Conclusions An electrochemical DNA biosensor based on polypyrrole/multiwalled carbon nanotubes modified electrode and biotin-avidin system fixed DNA probes could be developed for the fast detection of salmonella." @default.
- W2360949191 created "2016-06-24" @default.
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- W2360949191 date "2011-01-01" @default.
- W2360949191 modified "2023-09-26" @default.
- W2360949191 title "DNA electrochemical biosensor based on polypyrrole/multiwalled carbon nanotubes composite film" @default.
- W2360949191 hasPublicationYear "2011" @default.
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