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- W2360956556 abstract "Objective To detect the genes encoding allergens from dust mites in the dust screens of air conditioning systems. Methods Twenty dust samples were collected from air conditioning filters in residences. After extraction of genomic DNA, genes encoding group 1, 2, and 3 allergens (ProDer f 1, ProDer p 1, Der f 2, Der p 2, Der f 3, Der p 3) of Dermatophagoides farinae and Dermatophagoides pteronyssinus were amplified with PCR using specific primers. The purified PCR products were inserted into a vector, pUCm-T, and transformed into Escherichia coli DH-5α competent cells. Transformants were plated on LB Amp+ and incubated overnight at 37°C. A monoclonal colony was randomly selected for PCR verification, positive clone sequencing, and sequence alignment. Results Of 20 total samples, genes encoding allergens produced by dust mites were detected in 17. Of these, 6 contained ProDer f 1, ProDer p 1, Der f 2, and Der p 2; the remaining positive samples were found to contain one or more genes encoding allergens produced by D. farinae and/or D. pteronyssinus. Conclusion Air conditioner filters in human dwellings in the Wuhu area potentially contain group 1 and 2 allergens from D. farinae and D. pteronyssinus, both of which are associated with allergic diseases." @default.
- W2360956556 created "2016-06-24" @default.
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- W2360956556 date "2013-01-01" @default.
- W2360956556 modified "2023-09-23" @default.
- W2360956556 title "Detection of genes encoding allergens from dust mites in air filters of air conditioning systems" @default.
- W2360956556 hasPublicationYear "2013" @default.
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