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- W2360967649 abstract "Porcine reproductive and respiratory syndrome(PRRS) is one of the most important diseases that affect the swine industry nowadays.The GP5 gene of PRRS virus was cloned and then subcloned into adenovirus shuttle vectors pAdTrack-CMV,and the transfer vector pAdTrack-CMV/GP5 was constructed.After being linearized,the pAdTrack-CMV/GP5 was transformed into the E.coli strain BJ5183 cells and the recombinant supercoiled adenoviral vector pAdEasy-1 was constructed in the cells.After the inverted terminal repeat(ITR) sequence of pAdEay-1 was exposed fully by restriction enzyme digestion,the resultant recombinant was transfected into AD-293 with lipoplast.The current results showed that a stable and safety replication defective recombinant adenovirus AD-GP5 was produced successfully.The GP5 was transcribed and expressed,which was confirmed by a fluorescence microscope." @default.
- W2360967649 created "2016-06-24" @default.
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- W2360967649 date "2008-01-01" @default.
- W2360967649 modified "2023-09-23" @default.
- W2360967649 title "Construction and characterization of recombinant adenovirus with porcine reproductive and respiratory syndrome virus GP_5" @default.
- W2360967649 hasPublicationYear "2008" @default.
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