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- W2360994562 abstract "Objective:To establish a bi-phase system for the culture of primary B-lineage precursor leukemia cells in vitro and attempt to identify aggressive leukemic clone.Method:An immortalized human bone marrow stromal cell line,HS-5,was employed as a feeder-layer to establish a bi-phase culture system for the cultivation B-lineage precursor acute lymphoblastic leukemia cells.Meanwhile,the culture system was evaluated by drug toxicity assay and apoptotic testing.Result:The bi-phase culture system can tolerate the cell toxic drug induced apoptosis.Survival of B-lineage precursor lymphocytic leukemia cells in 7 of 8 cases of studied samples can be maintained with this bi-phase culture system.In contrast,the remaining 1 case of sample can survive regardless of the existing of feeder-layer.Conclusion:The bi-phase culture system is qualified for the cultivation of primary Pre-B leukemia cells but this survival supporting capability is varied from individual leukemia samples.Through the bi-phase culture,aggressive leukemic sample might be identified." @default.
- W2360994562 created "2016-06-24" @default.
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- W2360994562 date "2006-01-01" @default.
- W2360994562 modified "2023-09-25" @default.
- W2360994562 title "Establishment and evaluation of a bi-phase culture system for the culture of primary B-lineage precursor leukemic blasts in vitro" @default.
- W2360994562 hasPublicationYear "2006" @default.
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