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- W2360998909 abstract "[Objective] To clone and express 2-O-sulfatase gene from Flavobacterium heparinum.[Method] The 2-O-sulfatase gene was amplified with PCR from the genomic DNA of Flavobacterium heparinum,and it was linked into expression plasmid pET-28a after sequencing,then the recombined plasmid was expressed in E.coli BL21(DE3).[Result] The recombined pET-28a(+)with the PCR product of 2-O-sulfatase which was confirmed by DNA sequencing was successfully constructed.The expression product of the recombined plasmid pET-28a in E.coli BL21(DE3) was tested by SDS-PAGE electrophoresis.[Conclusion] The 2-O-sulfatase gene from Flavobacterium heparinum has been cloned and expressed successfully.These findings provide a framework that enables the use of 2-O-sulfatase as a tool for the determination of GAG and its degradation products' fine structure." @default.
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- W2360998909 date "2011-01-01" @default.
- W2360998909 modified "2023-09-26" @default.
- W2360998909 title "Cloning and Expression of Flavobacterium heparinum 2-O-sulfatase" @default.
- W2360998909 hasPublicationYear "2011" @default.
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