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• W2361014265 abstract "Objective 12-0-tetradecanoylphorbol 13 acetate (TPA)plays an important role in precipitating cell differentiation for various tumor cells, especially leukemic cells. Changes of many genes may be involved in this process. The purpose of this study was to observe the relationship between the EGR1mRNA expression and cell differentiation during TPA induced K562 cell differentiation. Methods Incubation of human K562 cells in vitro was applied to cultivate K562 cells. The cells were treated in two different ways. K562 cells of experiment group were treated with TPA and those of control group were treated without TPA. Using morphology (Wright′s staining and NSE staining) and flow cytometry (FCM), the investigators observed the differentiation characteristics of K562 cells, cell cycle and the differentiation antigen expressions of CD 33 and CD 14 on cell membranes. RT PCR was carried out to assay EGR1 mRNA expression. Results After treated with TPA for 7d, the morphology of K562 cells obviously tended to mature differentiation, like monocytes. The differentiation rate of induced K562 cells was up to 95% in experiment group and 4 5% in control group, respectively. Using SPSS software, the above result showed statistical significance ( P 0 01). Using NSE staining, K562 cells showed positive reaction. Some of them were densely stained. The positive rate was up to 86%. More than half of the positive cells could be inhibited by NaF. The inhibiting rate of NaF was up to 58 72%, showing statistical difference when compared with that of control group. FCM analysis showed that most of K562 cells stimulated by TPA underwent G1/S phase cell cycle arrest. The composing rate of cell cycle in TPA treated group showed that(53 7 ±1 25)% of cells were at G 0+ G 1 phase and (44 3±1 32)% were at S phase ( P 0 05). The level of CD 33 expression on cell membranes was mildly decreased from 0 997% to 0 893% ( P 0 05).However, the level of CD 14 expression was significantly increased from 0 049% to 0 387%( P 0 05).Conclusion K562 cells could express EGR1mRNA during TPA induced differentiation, which suggested that EGR1mRNA might participate in the process of K562 cells differentiating into monocyte/macrophages, and might play an important role in precipitating and maintaining cell differentiation for leukemic cells." @default.
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• W2361014265 date "2004-07-01" @default.
• W2361014265 modified "2023-09-25" @default.
• W2361014265 title "[EGR-1 mRNA expression during 12-0-tetradecanoylphorbol-13-acetate-induced K562 cell differentiation]." @default.
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