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- W2361058222 abstract "Objective To investigate the application of Anti-Keratin auto Antibody (AK auto Ab) on pure culture of melanocytes in serum-free medium and find a safe and simple method to culture melanocytes in vitro. Methods Epidermal cell suspension was produced by routine methods,then the cells was cultured in KC-SFM supplment with AK auto Ab,bFGF and BPE.The culture medium was renewed after 48 hours, Later the changing medium only contained bFGF and BPE. Results AK auto Ab could selectively kill the keratinocytes.Pure melanocytes could be produced in primary culture in KC-SFM supplment with AK auto Ab, bFGF and BPE. Conclusion We could replace the medium containing TPA and serum with a defined medium containing AK auto Ab, bFGF and BPE. The safety of transplantation of the melanocytes grown with AK auto Ab was discussed." @default.
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- W2361058222 date "2005-01-01" @default.
- W2361058222 modified "2023-09-23" @default.
- W2361058222 title "Application of Anti-Keratin auto Antibody on pure culture of melanocytes in serum-free medium" @default.
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