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- W2361073661 abstract "Three linearized DNA fragments without selectable marker gene and vector backbone were constructed containing phyA gene with regulation sequences(UPN) or T-DNA borders(T-UPN) or the plasmid borders(V-UPN). The linearized genes without selectable marker and vector backbone are safe in biology, they were transformed into maize inbred lines respectively via pollen-tube pathway. PCR amplification for three transformants T1 seeds showed different positive rate, UPN was 0.44%, T-UPN was 2.2% and V-UPN was 1.8%. The results also showed that the ratio of successful transformant by pollen-tube pathway was related to the location of seeds and the time after pollination but not to the lines of maize. The results of phytase activity assays demonstrated that the phyA II gene has been expressed at protein levels in the transgenic maize." @default.
- W2361073661 created "2016-06-24" @default.
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- W2361073661 date "2008-01-01" @default.
- W2361073661 modified "2023-09-22" @default.
- W2361073661 title "Transconduct phyA II Gene into Maize Inbred Lines and the Elementary Apprise of Transgenic Progency" @default.
- W2361073661 hasPublicationYear "2008" @default.
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