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- W2361094023 abstract "OBJECTIVE To develop an HPLC method for determination of dextroisomer in Lamivudine.METHODS A cyclobond Ⅰ2000 column(250 mm×4.6 mm,5 μm)was used with the mobile phase of methanol-0.1 mol·L-1 ammonium acetate(5:95)at a flow rate of 0.8 ml·min-1 and the temperature of the column was 10℃-30℃ with a detective wavelength at 270 nm.RESULTS The separation of both enantiomers of Lamivudine could be achieved baseline resolution.The calibration curve for Lamivudine dextroisomer exhibited an excellent linear relationship with a correlation coefficient of 0.9995 in concentration range of 0.5-5.0 μg·ml-1.The detection limit was 1.5 ng for lamivudine dextroisomer.CONCLUSION The method is accurate,simple,quick and suitable for determination of Lamivudine dextroisomer." @default.
- W2361094023 created "2016-06-24" @default.
- W2361094023 creator A5072093498 @default.
- W2361094023 date "2009-01-01" @default.
- W2361094023 modified "2023-09-23" @default.
- W2361094023 title "Determination of dextroisomer in Lamivudine by HPLC" @default.
- W2361094023 hasPublicationYear "2009" @default.
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