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- W2361119042 abstract "To establish a simple,sensitive,accurate and rapid detection method for bifidobacteria.Molecular beacon probe and primers were designed and sythesized according to the conserved gene of Bifidobacterium in GenBank,and then reaction parameters of real-time PCR were optimized.For Real-Time PCR and molecular beacon detection for bifidobacteria,the intra-assay and inter-assay coefficient of variation were both less than 5%,indicating good reproducibility of this method,and no non-specific amplification was observed either.Compared with conventional PCR,this method is more sensitive and faster,and the detection limit was 5.7fg/PCR for bifidobacteria DNA.A linear standard curve was obtained between 2×108CFU/mL and 2×104CFU/mL(R0.97).The method of Real-time PCR and molecular beacon detection for bifodobacteria has many advantages,such as being sensitive,specific,simple and fast,and this method can be used in situ detection of bifidobacteria quantitatively." @default.
- W2361119042 created "2016-06-24" @default.
- W2361119042 creator A5078765873 @default.
- W2361119042 date "2007-01-01" @default.
- W2361119042 modified "2023-09-22" @default.
- W2361119042 title "Establishment of Real-Time PCR and Molecular Beacon Detection Method for Bifidobacteria" @default.
- W2361119042 hasPublicationYear "2007" @default.
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