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- W2361151137 abstract "OBJECTIVE:To study the effect of EGF(epidermal growth factor) on migration behavior of A549 cells in the direct-current electric fields.METHODS:The A549 cells were divided into 4 groups according to whether applying EGF or direct-current electric fields.The movement images were taken per 15 min within 2 hours by micro-videotape system.Total length of cell displacement and average directional cosine γ were measured.The data of results were analyzed statistically.Western blot method was used to determine expression of p-Akt and p-Stat 3 protein.RESULTS:Cells in group A and B unexposed to the electric fields showed random movements during 2 hours.Cells in group C and D exposed to the electric fields migrate to the cathode direction.The average directional cosineγ of the cells in group D was larger than that in other groups 0.83±0.03,P0.05.The total length of cell displacement in group D was longer than that in other groups also(63±16.9) μm,P0.05.The expression of p-Akt and p-Stat 3 protein was increased with the treatment of EGF and electric fields.CONCLUSIONS:Electric field is able to induce the cathodal directed migration of the A549 cells and moreover the EGF can enhance this directed migration.We believed that Akt and Stat signal conduction path might play a very important role in the directed migration of the A549 cells." @default.
- W2361151137 created "2016-06-24" @default.
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- W2361151137 date "2008-01-01" @default.
- W2361151137 modified "2023-09-26" @default.
- W2361151137 title "Enhancement of EGF to the directed migration of cultured A549 cells in direct-current electric fields" @default.
- W2361151137 hasPublicationYear "2008" @default.
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