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- W2361153520 abstract "This paper reports the effects ofthe component of growth medium,growth phase of mycelium,lytic temperature,and components of regeneration medium on protoplast formation and regeneration of S.qingfengmy-ceticus and S.hygroscopicus var.jinggangen-sis. The results shows that 1) My-celia grown on the media supplemen-ted with appropriate amount of gly-cine and/or sucrose increase the susceptibility to lysozyme.2) Regeneration rate of protoplasts from exponential phase mycelium is much higherthan that of stationary phase my-celium 3) Optimal lytic temperature is 28℃ for S.qingfengmyceticusand 32℃ for S.hygroscopicus var.jing-gangensis.4) Protoplast regenerationis markedly affected by the concentration of hypertonic stablizer in theregeneration medium. The possible mechanisms of theaction of glycine and sucrose on lysozy-me susceptibility to streptomycetes cellwall and the possible reasons of theoccurrence of different ratio of osmotic unstable protoplasts in differentstreptomycetes protoplast preparationare discussed." @default.
- W2361153520 created "2016-06-24" @default.
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- W2361153520 date "1984-01-01" @default.
- W2361153520 modified "2023-09-25" @default.
- W2361153520 title "FACTORS AFFECTING PROTOPLAST FORMATION AND REGENERATION OF STREPTOMYCETES" @default.
- W2361153520 hasPublicationYear "1984" @default.
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