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- W2361169985 abstract "A simple isolation and purification method for arachidonic acid (5,8,11,14eicosatetraenoic acid, AA) from microbial lipids is presented. At first, AA was concentrated by urea inclusion reaction from mixed fatty acids made from microbial lipids, and then esterified with BF3methanol. AA was successfully isolated and purified with reversedphase high performance liquid chromatography on a μBondapakTM C18 preparative column (30 mm id×300 mm, 15 μm), using methanolwater (95∶5 in volume ratio) as the mobile phase at a flow rate of 5 mL/min and detected by a 410 Waters differential refractometer. After urea inclusion reaction the sample was refined and the content of AA was greatly increased. Finally, the purity of AA reached 99% from the initial content of 38%. The analysis was carried out by gas chromatography/mass spectrometry (GC/MS)." @default.
- W2361169985 created "2016-06-24" @default.
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- W2361169985 date "2003-01-01" @default.
- W2361169985 modified "2023-09-24" @default.
- W2361169985 title "Isolation and Purification of Arachidonic Acid in Microbial Lipids by High Performance Liquid Chromatography" @default.
- W2361169985 hasPublicationYear "2003" @default.
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