Matches in SemOpenAlex for { <https://semopenalex.org/work/W2361181960> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2361181960 abstract "Objective To clone and sequence the cDNA encoding actin gene from the roots of Codonopsis pilosula.Methods Degenerate primers were designed based on the conserved sequences of the actin gene from other plants.Total RNA was extracted from the roots of C.pilosula.Actin gene fragment was obtained by reverse transcription polymerase chain reaction(RT-PCR) and PCR products are sub-cloned into pMD19-T Simple vector.The positive clone identified by PCR was sequenced.Results The sequencing result revealed that the actin gene fragment from the roots of C.pilosula contained about 603 bp,encoding 200 amino acids.Sequence analysis suggested that the nucleotide sequence and the translated amino acid sequence shared over 78% and 90% of homology respectively with actin gene sequences from other higher plants.Conclusion It is the first report that a novel actin gene is cloned from roots of C.pilosula.This work lays a foundation for application to actin gene." @default.
- W2361181960 created "2016-06-24" @default.
- W2361181960 creator A5079050618 @default.
- W2361181960 date "2011-01-01" @default.
- W2361181960 modified "2023-09-23" @default.
- W2361181960 title "Cloning and sequence analysis of actin gene fragment from roots of Codonopsis pilosula" @default.
- W2361181960 hasPublicationYear "2011" @default.
- W2361181960 type Work @default.
- W2361181960 sameAs 2361181960 @default.
- W2361181960 citedByCount "0" @default.
- W2361181960 crossrefType "journal-article" @default.
- W2361181960 hasAuthorship W2361181960A5079050618 @default.
- W2361181960 hasConcept C104317684 @default.
- W2361181960 hasConcept C121050878 @default.
- W2361181960 hasConcept C153911025 @default.
- W2361181960 hasConcept C165525559 @default.
- W2361181960 hasConcept C167625842 @default.
- W2361181960 hasConcept C187882448 @default.
- W2361181960 hasConcept C19924922 @default.
- W2361181960 hasConcept C199360897 @default.
- W2361181960 hasConcept C41008148 @default.
- W2361181960 hasConcept C54355233 @default.
- W2361181960 hasConcept C61053724 @default.
- W2361181960 hasConcept C84148353 @default.
- W2361181960 hasConcept C86803240 @default.
- W2361181960 hasConceptScore W2361181960C104317684 @default.
- W2361181960 hasConceptScore W2361181960C121050878 @default.
- W2361181960 hasConceptScore W2361181960C153911025 @default.
- W2361181960 hasConceptScore W2361181960C165525559 @default.
- W2361181960 hasConceptScore W2361181960C167625842 @default.
- W2361181960 hasConceptScore W2361181960C187882448 @default.
- W2361181960 hasConceptScore W2361181960C19924922 @default.
- W2361181960 hasConceptScore W2361181960C199360897 @default.
- W2361181960 hasConceptScore W2361181960C41008148 @default.
- W2361181960 hasConceptScore W2361181960C54355233 @default.
- W2361181960 hasConceptScore W2361181960C61053724 @default.
- W2361181960 hasConceptScore W2361181960C84148353 @default.
- W2361181960 hasConceptScore W2361181960C86803240 @default.
- W2361181960 hasLocation W23611819601 @default.
- W2361181960 hasOpenAccess W2361181960 @default.
- W2361181960 hasPrimaryLocation W23611819601 @default.
- W2361181960 hasRelatedWork W2219097333 @default.
- W2361181960 hasRelatedWork W2356906862 @default.
- W2361181960 hasRelatedWork W2358989811 @default.
- W2361181960 hasRelatedWork W2361881337 @default.
- W2361181960 hasRelatedWork W2361884362 @default.
- W2361181960 hasRelatedWork W2369467474 @default.
- W2361181960 hasRelatedWork W2373109841 @default.
- W2361181960 hasRelatedWork W2374341016 @default.
- W2361181960 hasRelatedWork W2377056028 @default.
- W2361181960 hasRelatedWork W2377373861 @default.
- W2361181960 hasRelatedWork W2378481901 @default.
- W2361181960 hasRelatedWork W2379374324 @default.
- W2361181960 hasRelatedWork W2383577522 @default.
- W2361181960 hasRelatedWork W2383580154 @default.
- W2361181960 hasRelatedWork W2385223256 @default.
- W2361181960 hasRelatedWork W2388005596 @default.
- W2361181960 hasRelatedWork W2388715700 @default.
- W2361181960 hasRelatedWork W2393696577 @default.
- W2361181960 hasRelatedWork W3025617812 @default.
- W2361181960 hasRelatedWork W3196310640 @default.
- W2361181960 isParatext "false" @default.
- W2361181960 isRetracted "false" @default.
- W2361181960 magId "2361181960" @default.
- W2361181960 workType "article" @default.