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- W2361207009 abstract "Objective: To prepare demineralized bone matrix(DBM) and observe the potential of using DBM as a scaffold for bone tissue engineering.Methods: DBM of rabbit was prepared based on the Urist's method,and bone marrow stromal cells(BMSCs) of rats and DBM were combined cultured in vitro for 7 days.The composite DBM/BMSCs were implanted into the bilateral hind limb muscle pocket of ICR mice.DBM implant alone was used as control.X-ray examination was performed on the 7th and 28th days after operation.The implanted composite were harvested on the 7th,14th,and 28th days post operation,and histological changes were observed and calcium contents were assessed.Results: In experimental group,the osteoblasts appeared 7 days post operation,the quantities of osteoblasts and calcium contents of the implant increased with the lapse of time,and comparatively matured bone tissue appeared 28 days after operation.In control group,only few osteoblasts appeared 14 days postoperation,and on the 28th day after operation,only a small quantity of osteoid appeared,with a very low level of calcium contents.Conclusion: The DBM/BMSCs compound had favorable abilities of ectopic osteogenesis.The DBM scaffold could be a good scaffold of bone tissue engineering." @default.
- W2361207009 created "2016-06-24" @default.
- W2361207009 creator A5078137307 @default.
- W2361207009 date "2012-01-01" @default.
- W2361207009 modified "2023-09-25" @default.
- W2361207009 title "Experimental Study of Ectopic Osteogenesis of DBM/BMSCs Compound" @default.
- W2361207009 hasPublicationYear "2012" @default.
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