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- W2361224346 abstract "R40 S.cerevisiae of respiration defect type was screened out by UV mutagensis,and then the preparation and regeneration of protoplast with R40 S.cerevisiae and P01 Pachysolen tannophilus as parent strains were carried out and the effects of enzyme concentration,enzymatic hydrolysis temperature,and enzymatic hydrolysis time on the preparation and regeneration of protoplast were investigated.The results showed that as enzyme concentration was 2 % and enzymatic hydrolysis time was 1h,the preparation rate and regeneration rate of protoplast for Angel R40 S.cerevisiae could reach up 96.1 % and 12.5 % respectively at 35 ℃,and the preparation rate and regeneration rate of protoplast for P01 Pachysolen tannophilus could reach up 97.2 % and 10.1 % respectively at 30 ℃." @default.
- W2361224346 created "2016-06-24" @default.
- W2361224346 creator A5083351395 @default.
- W2361224346 date "2011-01-01" @default.
- W2361224346 modified "2023-09-25" @default.
- W2361224346 title "Preparation and Regeneration of Protoplast for Angel S.cerevisiae and Pachysolen tannophilus" @default.
- W2361224346 hasPublicationYear "2011" @default.
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