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- W2361234163 abstract "The BALB/c mice were immunized with purified recombinant protein infectious pancreatic necrosis virus(IPNV) VP3.Two hybridoma cell lines against the VP3 protein were obtained by screening with the indirect ELISA and limiting dilution assay,identification of monoclonal antibodies by western-blot and indirect immunofluorescence assay.These two cell lines named 2F1、4A7 can stably secrete specific antibodies.Two hybridoma cell lines were identified to be IgG1 subtype.Western-blot analysis showed that the two McAbs could react with IPNV VP3 recombinant protein,and the two McAbs could combine with IPNV,which was certified by indirect immunofluorescence,and two McAbs had no reactive capability withVHSV,SVCV and HRV,specificity only for IPNV." @default.
- W2361234163 created "2016-06-24" @default.
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- W2361234163 date "2013-01-01" @default.
- W2361234163 modified "2023-09-23" @default.
- W2361234163 title "Development of monoclonal antibodies against VP3 protein of infectious pancreatic necrosis virus" @default.
- W2361234163 hasPublicationYear "2013" @default.
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