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- W2361235744 abstract "Objects To investigate the different effects of mesenchymal stem cells(MSCs)and hematopoietic stem cells(HSCs)on hepatic differentiation.Methods MSCs from rat bone marrow were isolated and cultured by standard methods.Using Thy-1.1 as the marker of rat HSCs,Thy-1.1+ cells were isolated and purified by magnetic activated cell sorting(MACS).The optimal condition of isolation,cultivation and induction of MSCs and HSCs were investigated.Morphology,RT-PCR and immunocytochemistry were used to identify the hepatic differentiation grade.Results The purity and viability of Thy-1.1 + cells were 94.20 %,99.62 % after MACS.MSCs exhibited round shape after differentiation,instead of fibroblast-like morphology before differentiation.Albumin mRNA and protein were expressed positively in MSCs,and no alpha-fetoprotein(AFP)was found.HSCs were polygonal in shape after differentiation.The expression of albumin signal decreased and AFP signal increased.Conclusions Highly purified Thy-1.1 + cells can be obtained from rat bone marrow by using MACS,and the viability of the cells was not impaired.Both MSCs and HSCs have hepatic differentiation capabilities.However,their capabilities were not the same.MSCs can differentiate into mature hepatocyte-like cells,and do not express early hepatic specific genes,while Thy-1.1 + cells were inclined to differentiate into hepatic stem cell-like cells,with an increased AFP expression and a decreased albumin signal." @default.
- W2361235744 created "2016-06-24" @default.
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- W2361235744 date "2006-01-01" @default.
- W2361235744 modified "2023-09-23" @default.
- W2361235744 title "Comparative Study of in vitro Hepatic Differentiation Capability of Two Different Stem Cell Subsets Derived from Bone-marrow" @default.
- W2361235744 hasPublicationYear "2006" @default.
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