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- W2361240571 abstract "Aim:To obtain the high titer recombinant adenovirus expressing human HA117 gene.Methods:The cDNA of HA117 gene was subcloned into the shuttle plasmid pAdTrack-CMV and then clone the homologous recombinant adenovims genomie plasmid pAdeasy in bacteria.We tranfected the DNA of identified recombinant plasmid into 293 ceils via liposome,and then did the package and amplification of adenovims, determined by fluorescence microscope.The expression of HA117 was monitored by RT-PCR,fluorescence and flow eytometry in infected K562 cells.Results:High titer recombined adnovirus carring HA117 gene was successfully obtained.The titer of the recombinant adenovims was 8.3×1011pfu/mL,and The expression of HA117 gene was observed. The specific expression of HA117 gene was identified by RT-PCR in K562 cell after infection with Ad-HA117.Conclusion:High titer recombined adnovirus carring HA117 gene was successfully obtained.Infected K562 cell by recombinant adenovims highly expressed HA117 gene." @default.
- W2361240571 created "2016-06-24" @default.
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- W2361240571 date "2009-01-01" @default.
- W2361240571 modified "2023-09-25" @default.
- W2361240571 title "Obtaining of high titer recombined adenovirus carring HA117 gene and its expression in K562 cells" @default.
- W2361240571 hasPublicationYear "2009" @default.
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