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- W2361242486 abstract "The same MOX promoter fragments of Hansenula yeast host genomic DNA and fermentation products genomic DNA are taken as specific probes.Then the specific probes with digoxigenin labeled are hybridized and been color with host genome DNA fixed in the hybrid membrane and fermentation products genome DNA.The copy number size of foreign gene in fermentation product according to the color depth can be determined.The results show that the copy number of HBsAg foreign gene in fermentation product is not less than 30.The method has such advantages as better detecting sensitivity,stronger specificity and good safety.Therefore,it can be used in qualitative detection of copy number size of HBsAg foreign gene in fermentation product of recombinant Hansenula yeast." @default.
- W2361242486 created "2016-06-24" @default.
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- W2361242486 date "2012-01-01" @default.
- W2361242486 modified "2023-09-25" @default.
- W2361242486 title "Hybridization Detection of Copy Number of HBsAg Foreign Gene in Fermentation Products of Recombinant Hepatitis B(Hansenula)" @default.
- W2361242486 hasPublicationYear "2012" @default.
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