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- W2361288258 abstract "A novel non-chromatographic ion-exchange adsorption technique was developed to purify polyclonal antibody against Vibrio parahaemolyticus from rabbit serum using carboxylated superparamagnetic nanobeads.pH and NaCl concentration of adsorption and desorption buffers and Tween-20 concentration in washing buffer were studied,and the developed method was compared with caprylic acid-ammonium sulfate precipitation and SPA affinity chromatography.The results showed that the polyclonal antibody could be completely adsorbed by magnetic beads in 0.01 mol/L phosphate buffer(pH 6.0) and desorbed in 0.01 mol/L phosphate buffer(pH 8.0) containing 0.3 mol/L NaCl,and unadsorbed proteins were removed by washing twice with washing buffer containing 0.1% Tween-20.After the purification,the polyclonal IgG purity was 82.6%,the recovery yield 58.0%,and the titer 1/64000.In total,10 mL of rabbit serum could be treated by 1 g of magnetic beads,and the whole process could be finished within 1 h.The method has obvious advantages compared with caprylic acid-ammonium sulfate precipitation and SPA affinity chromatography,thereby presenting a promising application prospect." @default.
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- W2361288258 date "2011-01-01" @default.
- W2361288258 modified "2023-09-24" @default.
- W2361288258 title "Ion-Exchange Purification of Polyclonal Antibody from Rabbit Serum Using Magnetic Beads" @default.
- W2361288258 hasPublicationYear "2011" @default.
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