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- W2361432825 abstract "Objective To clone and analyze the molecular ev olut ion of hemagglutinin gene of avian influenza virus (AIV) subtype H 5N 1. Methods The full-length cDNA of hemagglutinin (HA) gene was amplifie d from 2 avian influenza virus isolates (H 5N 1) by RT-PCR,cloned into pUCm- T vector and sequenced. Results The cloned HA gene,at a full-length of 1 731 bp,encoded 56 8 amino acids.The homologies of nucleotide and amino acid sequences of HA genes of the isolates to those of other 10 strains of AIV subtype H5 were 80.5%-99.2% and 89.4%-98.6% respectively.There were 5 basic amino acids inserted into th e cleavage sites of HA gene of highly pathogenic AIV. Conclusion The study laid a foundation of development of recombinant av ian influenza vaccine.Meanwhile,the analysis on molecular evolution indicated th e relationship between various strains of AIV." @default.
- W2361432825 created "2016-06-24" @default.
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- W2361432825 date "2004-01-01" @default.
- W2361432825 modified "2023-09-25" @default.
- W2361432825 title "Cloning and Analysis of Molecular Evolution of Hemagglutinin Gene of Avian Influ enza Virus Subtype H_5N_1" @default.
- W2361432825 hasPublicationYear "2004" @default.
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