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- W2361497249 abstract "Objective To optimize the method for quantifying microRNAs in different experimental purposes and conditions by comparing two real-time quantitative reverse transcription-polymerase chain reaction(RT-qPCR) methods.Methods We isolated the total RNA from the SD rat articular cartilage at postnatal day 21 and day 42 with TRIzol reagent.The RT reactions were performed by stem-loop primers and the universal primer of miRNA detection kit respectively;then real time PCR was performed to test the expressions of rno-miR-15b,rno-miR-16,rno-miR-195 and rno-miR-497.In addition,the total RNAs in human plasma were isolated by using TRI Reagent BD(MRC,TR126) according to the instructions by the manufacturer with two different RT-qPCR methods to quantify the expression of has-miR-16.Results The expression change of these miRNAs was of the same increase trend by the two different RT-qPCR methods,which accorded with the results of our Solexa sequencing.The results of plasma demonstrated that stem-loop RT-qPCR method was relatively sensitive.Conclusion Stem-loop RT-qPCR method shows the advantages of testing a few important miRNAs while the detection kit method applies to screening a large number of miRNAs." @default.
- W2361497249 created "2016-06-24" @default.
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- W2361497249 date "2013-01-01" @default.
- W2361497249 modified "2023-09-23" @default.
- W2361497249 title "Technical analysis for detection and quantification of microRNAs by two real-time quantitative reverse transcription methods" @default.
- W2361497249 hasPublicationYear "2013" @default.
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