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- W2361503898 abstract "Objective To develop a HPLC assay for the content determination of rutoside in the extracts of wolfberry from mi crowave extraction. Methods HPLC method:Column:Dikma Kromasil C18 (4.6 mm × 200 mm,5 μm). The column temperature was maintained at 25 ℃. Mobile phase:methanol-0.3% phosphoric acid (37 :63),with flow rate 1 ml.min-1. UV detector wavelength:257 nm. Inject volume:5 μL. The established HPLC assay for the quantitative determination of rutoside was fully evaluated. Then,three different extraction methods including reflux extraction,ultrasonic extraction,and microwave extraction were applied to evaluate the impaction of different extraction methods on the content determination of rutoside. Results The HPLC assay for the rutoside was linear within 0.004～ 0.11 mg/ml (R2=0.9998). The average recovery was 101.48%(n=5). Significant difference in the rutoside content was identified among the wolfberry from different producing areas. Extremely high difference in the rutoside content was identified between the real and fake wolfberry. Conclusion In current research,an accurate and reproducible HPLC method is developed and evaluated to determine the content of rutoside in the extracts of wolfberry. Current research provides valuable data for the establishment of the wolfberry quality standard." @default.
- W2361503898 created "2016-06-24" @default.
- W2361503898 creator A5087790946 @default.
- W2361503898 date "2010-01-01" @default.
- W2361503898 modified "2023-09-28" @default.
- W2361503898 title "The HPLC Assay for Content Determination of Rutoside in the Extracts of Wolfberry from Microwave Extraction" @default.
- W2361503898 hasPublicationYear "2010" @default.
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