Matches in SemOpenAlex for { <https://semopenalex.org/work/W2361519277> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2361519277 abstract "Objectives To express TSP6 of Cryptosporidium parvum in Escherichia coli BL21 and develop monoclonal antibodies(McAb) against rCpTSP6.Methods The recombinant plasmid pGEX-4T-1-TSP6 was transformed into E.coli BL21(DE3).Point-one mmol/L IPTG was used to induce the expression of recombinant TSP6.The rTSP6 was soluble in supernatant after sonication and further purified with Glutathione-Sepharose 4B.BALB/c mice were immunized with purified proteins and cell fusion was induced via the hybridoma technique.McAbs were screened by ELISA with limited dilution.The subtype and specificity of McAbs were identified by kit and Western blot analysis,respectively.Results Two hybridoma cell lines secreting monoclonal antibodies against rTSP6 were obtained.The subtype of both was IgG1.Western blot analysis demonstrated that both of the McAbs reacted strongly with corresponding recombinant proteins.Conclusion Two hybridoma cell lines secreting highly specific McAbs against rTSP6 have been established." @default.
- W2361519277 created "2016-06-24" @default.
- W2361519277 creator A5061867449 @default.
- W2361519277 date "2013-01-01" @default.
- W2361519277 modified "2023-09-22" @default.
- W2361519277 title "Development and identification of monoclonal antibodies against the recombinant tsp6 antigen of Cryptosporidium parvum" @default.
- W2361519277 hasPublicationYear "2013" @default.
- W2361519277 type Work @default.
- W2361519277 sameAs 2361519277 @default.
- W2361519277 citedByCount "0" @default.
- W2361519277 crossrefType "journal-article" @default.
- W2361519277 hasAuthorship W2361519277A5061867449 @default.
- W2361519277 hasConcept C104317684 @default.
- W2361519277 hasConcept C123894998 @default.
- W2361519277 hasConcept C147483822 @default.
- W2361519277 hasConcept C153911025 @default.
- W2361519277 hasConcept C159047783 @default.
- W2361519277 hasConcept C159654299 @default.
- W2361519277 hasConcept C179247698 @default.
- W2361519277 hasConcept C181199279 @default.
- W2361519277 hasConcept C203014093 @default.
- W2361519277 hasConcept C2776415932 @default.
- W2361519277 hasConcept C2777795018 @default.
- W2361519277 hasConcept C40767141 @default.
- W2361519277 hasConcept C542903549 @default.
- W2361519277 hasConcept C547475151 @default.
- W2361519277 hasConcept C55493867 @default.
- W2361519277 hasConcept C86803240 @default.
- W2361519277 hasConceptScore W2361519277C104317684 @default.
- W2361519277 hasConceptScore W2361519277C123894998 @default.
- W2361519277 hasConceptScore W2361519277C147483822 @default.
- W2361519277 hasConceptScore W2361519277C153911025 @default.
- W2361519277 hasConceptScore W2361519277C159047783 @default.
- W2361519277 hasConceptScore W2361519277C159654299 @default.
- W2361519277 hasConceptScore W2361519277C179247698 @default.
- W2361519277 hasConceptScore W2361519277C181199279 @default.
- W2361519277 hasConceptScore W2361519277C203014093 @default.
- W2361519277 hasConceptScore W2361519277C2776415932 @default.
- W2361519277 hasConceptScore W2361519277C2777795018 @default.
- W2361519277 hasConceptScore W2361519277C40767141 @default.
- W2361519277 hasConceptScore W2361519277C542903549 @default.
- W2361519277 hasConceptScore W2361519277C547475151 @default.
- W2361519277 hasConceptScore W2361519277C55493867 @default.
- W2361519277 hasConceptScore W2361519277C86803240 @default.
- W2361519277 hasLocation W23615192771 @default.
- W2361519277 hasOpenAccess W2361519277 @default.
- W2361519277 hasPrimaryLocation W23615192771 @default.
- W2361519277 hasRelatedWork W2196101296 @default.
- W2361519277 hasRelatedWork W2356252278 @default.
- W2361519277 hasRelatedWork W2357522056 @default.
- W2361519277 hasRelatedWork W2360091834 @default.
- W2361519277 hasRelatedWork W2361508593 @default.
- W2361519277 hasRelatedWork W2362323045 @default.
- W2361519277 hasRelatedWork W2368688748 @default.
- W2361519277 hasRelatedWork W2370383585 @default.
- W2361519277 hasRelatedWork W2372822509 @default.
- W2361519277 hasRelatedWork W2374579300 @default.
- W2361519277 hasRelatedWork W2378283768 @default.
- W2361519277 hasRelatedWork W2381046792 @default.
- W2361519277 hasRelatedWork W2382482751 @default.
- W2361519277 hasRelatedWork W2386060881 @default.
- W2361519277 hasRelatedWork W2386326538 @default.
- W2361519277 hasRelatedWork W2387738693 @default.
- W2361519277 hasRelatedWork W2388899694 @default.
- W2361519277 hasRelatedWork W2401817308 @default.
- W2361519277 hasRelatedWork W2978251081 @default.
- W2361519277 hasRelatedWork W1929147813 @default.
- W2361519277 isParatext "false" @default.
- W2361519277 isRetracted "false" @default.
- W2361519277 magId "2361519277" @default.
- W2361519277 workType "article" @default.