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- W2361617275 abstract "Objective To construct genetically modified neural stem cells (NSCs) from fetal rat spinal cord.Methods Ex vivo via isolation and propagation,NSCs and phenotype of induced differentiation were detected by immunocytochemistry with antibody to Nestin,NF-200,GFAP.Recombinant glial-cell derived neurotrophic factor (GDNF) was applied to perform trangenetic modification for NSCs,and results were identified with immunofluoresence detection and immunocytochemical stain.Quantitative analysis of the transgenetic expression was detected by using ELISA immunoabsorbing assay.Results Large quantity of neurospheres were obtained from fetal rat spinal cord,which could be induced to differentiate into neurons and astrocytes.Transgenetic stem cells could express exogenetic products of GFP and GDNF during the following 3 weeks,and expressive content maintained at (93.0± 6.5) ng/L or so.Conclusion NSCs derived from fetal spinal cord maintain the ability of self-renewal and multipotency differentiation into neural cellls,the same as the genetically modified NSCs.The genetic engineering NSCs will be beneficial to further research on transgenetic treatment for central nervous trauma and degenerative disease." @default.
- W2361617275 created "2016-06-24" @default.
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- W2361617275 date "2005-01-01" @default.
- W2361617275 modified "2023-09-23" @default.
- W2361617275 title "Transgenetic manipulation of GDNF for neural stem cell from fetal rat spinal cord" @default.
- W2361617275 hasPublicationYear "2005" @default.
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