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- W2361629268 abstract "Objective To investigate the interaction between Periostin (osteoblast-specific factor 2) and SFRP 2 (Secreted frizzled-related protein 2) in the formation of keloid. Methods HA-tagged fusion protein (HA- Periostin) expression vector was constructed, identified and transfected into human embryo kidney 293 (HEK293) cells alone or with Myc-tagged fusion protein (Myc-SFRP 2) expression vector pMCV-Myc-SFRP 2. The interaction between SFRP 2 and Periostin was detected by pull-down technique. Results Double restriction enzyme digestion showed that pCMV-HA-Periostin was constructed successfully. When Myc-SFRP 2 was immunoprecipitanted, HA-Periostin was identified. And the interaction between SFRP 2 and Periostin was found by GST pull-down and His pull-down technique. Conclusion The recombinant vector pCMV-HA-Periostin was constructed successfully. The interaction between SFRP 2 and Periostin could be identified by GST pull-down and His pull-down." @default.
- W2361629268 created "2016-06-24" @default.
- W2361629268 creator A5012562375 @default.
- W2361629268 date "2009-01-01" @default.
- W2361629268 modified "2023-09-23" @default.
- W2361629268 title "Experimental Study of Interaction between SERP 2 and Periostin in the Formation of Keloid" @default.
- W2361629268 hasPublicationYear "2009" @default.
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