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- W2361630975 abstract "OBJECTIVE:To determine the encapsulation efficiency(EE) of BSA liposome.METHODS:Liposome and free BSA were separated through DEAE-Sephadex A50 minicolumn centrifuge.The volume ratio of sample to dyestuff and reaction time were screened.EE was determined by minicolumn centrifuge combined with two-colorimetric coomassie brilliant blue protein assay/ELIASA(595 nm,450 nm) .RESULTS:The volume ratio of sample to dyestuff was 1 ∶ 3,and reaction time was 10 min.The average recovery rate was 99.56%(RSD2.73%) .The average recovery rate of blank liposome in minicolumn was 97.78% and separation efficiency was more than 90%.The EE of BSA liposome was 32.91%(n=3) .CONCLUSIONS:The method is rapid,effective,simple and feasible for the separation of free protein and liposome.Compared with common gel filtration column centrifuge,established method increases diluent fold of sample significantly and it is suitable for the EE determination of trace larger proteins molecule liposome." @default.
- W2361630975 created "2016-06-24" @default.
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- W2361630975 date "2011-01-01" @default.
- W2361630975 modified "2023-09-23" @default.
- W2361630975 title "Determination of Encapsulation Efficiency of BSA Liposome by Minicolumn Centrifuge Combined with Two-colorimetric Coomassie Brilliant Blue Protein Assay/ELIASA" @default.
- W2361630975 hasPublicationYear "2011" @default.
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