Matches in SemOpenAlex for { <https://semopenalex.org/work/W2361666546> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2361666546 abstract "Objective To construct and produce the vector of NT4-GFP-Ant recombinant adeno-associated virus(AAV).Methods The resulting gene of NT4-GFP-Ant was inserted into the EcoR I-BamH I site of vector plasmid pSSHG to construct the vector of NT4-GFP-Ant recombinant AAV.The recombinant AAV viral sock was packaged.Renal embryo 293 cell was co-transfected with the rAAV vector of plasmid pSSHG/ NT4-GFP-Ant, packaging plasmid pAAV/Ad and helper adenoviruspasmid pFG140.The neuroblastoma cell was transfected with rAAVand the supernatant was transfered into 293 cell.Results The combinant viral stock vector of plasmid pSSHG/ NT4-GFP-Ant was constructed successfully.The results of dot blot showed that we had obtained the rAAV stocks of high titre(1.46×10~(12)PFU/ml).The fluorescence can be observed in the 293 cell with the fluores- cence microscope.Conclusion The viral stock of rAAV NT4-GFP-Ant was constructed and obtained successfully and the cell experiment confirmed that it can secret macromolecular transmembrane reporting proteins.We can further investigate the feasibility of the macromoleeular functional proteins passing through the blood-brain-barrer into brain and treating central nervous systerm diseases." @default.
- W2361666546 created "2016-06-24" @default.
- W2361666546 creator A5056409589 @default.
- W2361666546 date "2007-01-01" @default.
- W2361666546 modified "2023-09-26" @default.
- W2361666546 title "The construction and production of NT4-GFP-Ant recombinant adeno-associated virus" @default.
- W2361666546 hasPublicationYear "2007" @default.
- W2361666546 type Work @default.
- W2361666546 sameAs 2361666546 @default.
- W2361666546 citedByCount "0" @default.
- W2361666546 crossrefType "journal-article" @default.
- W2361666546 hasAuthorship W2361666546A5056409589 @default.
- W2361666546 hasConcept C104317684 @default.
- W2361666546 hasConcept C142613039 @default.
- W2361666546 hasConcept C153911025 @default.
- W2361666546 hasConcept C159047783 @default.
- W2361666546 hasConcept C22744801 @default.
- W2361666546 hasConcept C2522874641 @default.
- W2361666546 hasConcept C2778107364 @default.
- W2361666546 hasConcept C32470452 @default.
- W2361666546 hasConcept C40767141 @default.
- W2361666546 hasConcept C54009773 @default.
- W2361666546 hasConcept C54355233 @default.
- W2361666546 hasConcept C86803240 @default.
- W2361666546 hasConcept C92087593 @default.
- W2361666546 hasConceptScore W2361666546C104317684 @default.
- W2361666546 hasConceptScore W2361666546C142613039 @default.
- W2361666546 hasConceptScore W2361666546C153911025 @default.
- W2361666546 hasConceptScore W2361666546C159047783 @default.
- W2361666546 hasConceptScore W2361666546C22744801 @default.
- W2361666546 hasConceptScore W2361666546C2522874641 @default.
- W2361666546 hasConceptScore W2361666546C2778107364 @default.
- W2361666546 hasConceptScore W2361666546C32470452 @default.
- W2361666546 hasConceptScore W2361666546C40767141 @default.
- W2361666546 hasConceptScore W2361666546C54009773 @default.
- W2361666546 hasConceptScore W2361666546C54355233 @default.
- W2361666546 hasConceptScore W2361666546C86803240 @default.
- W2361666546 hasConceptScore W2361666546C92087593 @default.
- W2361666546 hasLocation W23616665461 @default.
- W2361666546 hasOpenAccess W2361666546 @default.
- W2361666546 hasPrimaryLocation W23616665461 @default.
- W2361666546 hasRelatedWork W1983074066 @default.
- W2361666546 hasRelatedWork W2351625714 @default.
- W2361666546 hasRelatedWork W2352494708 @default.
- W2361666546 hasRelatedWork W2354011035 @default.
- W2361666546 hasRelatedWork W2356324463 @default.
- W2361666546 hasRelatedWork W2356960668 @default.
- W2361666546 hasRelatedWork W2357736139 @default.
- W2361666546 hasRelatedWork W2366668597 @default.
- W2361666546 hasRelatedWork W2366956645 @default.
- W2361666546 hasRelatedWork W2368066453 @default.
- W2361666546 hasRelatedWork W2373177379 @default.
- W2361666546 hasRelatedWork W2375423701 @default.
- W2361666546 hasRelatedWork W2378821125 @default.
- W2361666546 hasRelatedWork W2384710499 @default.
- W2361666546 hasRelatedWork W2390819680 @default.
- W2361666546 hasRelatedWork W2394405458 @default.
- W2361666546 hasRelatedWork W2413847679 @default.
- W2361666546 hasRelatedWork W2419432085 @default.
- W2361666546 hasRelatedWork W2420122227 @default.
- W2361666546 hasRelatedWork W2619225075 @default.
- W2361666546 isParatext "false" @default.
- W2361666546 isRetracted "false" @default.
- W2361666546 magId "2361666546" @default.
- W2361666546 workType "article" @default.