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- W2361685194 abstract "Aim To establish a reliable and practical rat cardiomyocyte apoptosis model in vitro and in vivo.Methods In vitro cardiomyocyte apoptosis model:the neonatal SD rat cardiomyocytes were primarily cultured for 24 hours and the culture medium was changed to Hank′s balance sodium medium,and the cells were treated with hypoxia for 30 minutes,and with normal oxygen for 2 hours.Then cells were fixed,staining with TUNEL and the apoptotic cells were measured;the total DNA was extracted and gel electrophoresis was employed for the observation of DNA ladder.In vivo cardiomyocyte apoptosis model: WKY rats about 150 g were anaesthetized,fixed,and ventilated with a rodent ventilator.A parasternal incision was made and myocardial ischemia was induced by passing a 6-0 silk suture beneath the left anterior descending artery.The suture was tightened over a piece of PE-20 tubing for 30 min and then released for 2 h.Detection of apoptotic cardiomyocytes was performed with TUNEL staining using an apoptosis detection kit,according to the manufacturer′s instructions.Results TUNEL staining positive cells were observed in hypoxia/reoxygenation-treated cardiomyocytes,and DNA ladders were obvious.In the ischemia/reperfusion treated WKY rate heart slices,TUNEL positive cells were observed compared with control group.Conclusion Hypoxia/reoxygen-induced cardiomyocyte apoptosis in vitro and ischemia/reperfusion-induced cell apoptosis in vivo are reliable models inducing apoptosis." @default.
- W2361685194 created "2016-06-24" @default.
- W2361685194 creator A5076130185 @default.
- W2361685194 date "2011-01-01" @default.
- W2361685194 modified "2023-10-17" @default.
- W2361685194 title "Establishment of the in vitro and in vivo cardiomyocyte apoptosis model" @default.
- W2361685194 hasPublicationYear "2011" @default.
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