Matches in SemOpenAlex for { <https://semopenalex.org/work/W2361716807> ?p ?o ?g. }
Showing items 1 to 53 of
53
with 100 items per page.
- W2361716807 abstract "Objective: To detect the reduction of rabbit NADP(H)-dependent retinal reductase(NDRR)on menadione,and to identify its reductive production.Methods: Rabbit-NDRR was expressed in BL21-AITM with 6×His-Tag.The recombinant protein was purified by affinity chromatography on Ni2+-NTA-agarose after the transformed cells were sonicatied.The kinetic parameters of rabbit-NDRR were determined by HPLC under anaerobic condition and high-concentration of sodium dodecylsulphate(SDS).The reductive production was identified by chemical synthesis,high performance liquid chromatography(HPLC)as well as fluorescent scanning.Results: The homogeneous enzyme was obtained on a single step by affinity chromatography from the supernatant of transformed cells,and its reductive activity of menadione was 18.6-fold of the supernatant of the empty-transformed cells and 7.3-fold of transformed cells.The Km and Vmax detected by HPLC were 30.7 and 0.046 μmoL/(min·mg)respectively.The retention time of the product menadiol produced by menadione and NaBH4 or reducted by the enzyme was identical,and the fluorescence spectrum of menadiol could be obtained after the chemical reaction of menadione and NaBH4.Conclusion: Rabbit-NDRR can reduct menadione and its reductive product is menadiol." @default.
- W2361716807 created "2016-06-24" @default.
- W2361716807 creator A5077661251 @default.
- W2361716807 date "2008-01-01" @default.
- W2361716807 modified "2023-09-23" @default.
- W2361716807 title "Rabbit Liver NADP(H)-Dependent Retinal Reductase Expressed in Escherichia coli: Reduction of Menadione and Product Identification" @default.
- W2361716807 hasPublicationYear "2008" @default.
- W2361716807 type Work @default.
- W2361716807 sameAs 2361716807 @default.
- W2361716807 citedByCount "0" @default.
- W2361716807 crossrefType "journal-article" @default.
- W2361716807 hasAuthorship W2361716807A5077661251 @default.
- W2361716807 hasConcept C134651460 @default.
- W2361716807 hasConcept C179247698 @default.
- W2361716807 hasConcept C179998833 @default.
- W2361716807 hasConcept C181199279 @default.
- W2361716807 hasConcept C185592680 @default.
- W2361716807 hasConcept C2781095387 @default.
- W2361716807 hasConcept C43617362 @default.
- W2361716807 hasConcept C55493867 @default.
- W2361716807 hasConceptScore W2361716807C134651460 @default.
- W2361716807 hasConceptScore W2361716807C179247698 @default.
- W2361716807 hasConceptScore W2361716807C179998833 @default.
- W2361716807 hasConceptScore W2361716807C181199279 @default.
- W2361716807 hasConceptScore W2361716807C185592680 @default.
- W2361716807 hasConceptScore W2361716807C2781095387 @default.
- W2361716807 hasConceptScore W2361716807C43617362 @default.
- W2361716807 hasConceptScore W2361716807C55493867 @default.
- W2361716807 hasOpenAccess W2361716807 @default.
- W2361716807 hasRelatedWork W118809219 @default.
- W2361716807 hasRelatedWork W1519480480 @default.
- W2361716807 hasRelatedWork W1542342471 @default.
- W2361716807 hasRelatedWork W1577378124 @default.
- W2361716807 hasRelatedWork W1657207262 @default.
- W2361716807 hasRelatedWork W1852459632 @default.
- W2361716807 hasRelatedWork W1942497725 @default.
- W2361716807 hasRelatedWork W1965591579 @default.
- W2361716807 hasRelatedWork W1980234135 @default.
- W2361716807 hasRelatedWork W1982144156 @default.
- W2361716807 hasRelatedWork W2007008215 @default.
- W2361716807 hasRelatedWork W2013784907 @default.
- W2361716807 hasRelatedWork W2036336537 @default.
- W2361716807 hasRelatedWork W2042373069 @default.
- W2361716807 hasRelatedWork W2055225624 @default.
- W2361716807 hasRelatedWork W2059435513 @default.
- W2361716807 hasRelatedWork W2065405901 @default.
- W2361716807 hasRelatedWork W2067438519 @default.
- W2361716807 hasRelatedWork W2106555133 @default.
- W2361716807 hasRelatedWork W2183762380 @default.
- W2361716807 isParatext "false" @default.
- W2361716807 isRetracted "false" @default.
- W2361716807 magId "2361716807" @default.
- W2361716807 workType "article" @default.