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- W2361761566 abstract "Objective: To investigate the feasibility that a new Poly-β-hydroxybutyrate(PHB) used as a scaffold in cartilage tissue engineering.Methods: Chondrocytes acquired from cartilage tissue of juvenile New Zealand white rabbit were proliferated.The chondro-cytes were seeded on the scaffold in experimental group.After cultured for two weeks in vitro,the complex was implanted under the skin of adult rabbit's back.Only the PHB scaffold without chondrocytes was implanted in control group.The surface of scaffold and the grow-ing situation of chondrocytes were observed in the examination with scanning electron microscopy.The specimens were dissected and examined macroscopically and histologically after 4,8 and 12 weeks.HE and Masson staining was performed to observe the formation of tissue engineering cartilage.Results: The examination of scanning electron microscope revealed that the pores of the scaffold were well-distributed.The shape of pores was irregular.The chondrocytes on the scaffold were proliferated well after two weeks' culture in vit-ro.The size of specimens obtained at different time in the experimental group was not obviously changed.The specimens in control group became smaller and smaller.HE and Masson staining revealed that the scaffold had been completely absorbed after 12 weeks.The mature cartilage tissue had been formed in the experimental group after 12 weeks.The fibrous connective tissue was formed finally instead of the scaffold in control group.Conclusions: This PHB scaffold can be used as the scaffold of cartilage tissue engineering." @default.
- W2361761566 created "2016-06-24" @default.
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- W2361761566 date "2013-01-01" @default.
- W2361761566 modified "2023-09-22" @default.
- W2361761566 title "The Construction of Tissue Engineering Cartilage with A New Poly-β-hydroxybutyrate Scaffold" @default.
- W2361761566 hasPublicationYear "2013" @default.
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