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- W2361775721 abstract "The cmpABCD genes of Synechococcus sp.PCC 7942 which encoded an ABC-type HCO3-transporter involved in inorganic carbon(Ci) transporting in cyanobacterial CO2 concentrating mechanism(CCM),was amplified by PCR.And the resulting cmpBCD fragment was cloned in plasmid pUC19 to generate plasmid pUC-HATH.By inserting an hygromycin B pho transferase(hpt) cassette into the coding region of operon gene cmpABCD +1 508~+1 509 district in pUC-HAT,the homogenetic integration plasmid vector pUC-HATH was constructed and used to transform the Synechococcus sp.PCC 7942 wild-type strain,and a mutant with resistance to hygromycin B was obtained.PCR analysis of the genomic DNA from the resulting mutant indicated that the appropriate deletion and insertion indeed had occurred.The cell growth of the mutant didn't show quite different from those of the wild-type strain in improved BG-11 culture medium,which suggested that inactivation of cmpABCD genes in Synechococcus.sp.PCC7942 had little effect on the HCO3-transport activity." @default.
- W2361775721 created "2016-06-24" @default.
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- W2361775721 date "2006-01-01" @default.
- W2361775721 modified "2023-09-23" @default.
- W2361775721 title "Insertional Inactivation of cmpABCD Genesin Synechococcus sp.PCC7942" @default.
- W2361775721 hasPublicationYear "2006" @default.
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