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- W2361775969 abstract "Regeneration is a vital physiological process in Apostichopus japonicus,and was considered to a good model for organ and tissues culture in vitro.In order to further understand the function of some key molecules in this process,the tropomyosin gene of A.japonicus was cloned and expressed.The full-length of tropomyosin gene was 1203bp including a 105bp of 5' untranslated region (UTR),a 240bp of 3' UTR and a 855bp of open reading frame (ORF) encoding a polypeptide of 284 amino acids.The estimated molecular mass of tropomyosin gene is 33.27kDa,and the theoretical isoelectric point is 4.56.The recombinant pET-Trp protein was successfully expressed in E.coli.The recombinant protein pET-Trp had clearly visible band in 38kDa.The polyclonal antibody could specifically bind to the recombinant TRP by Western blot." @default.
- W2361775969 created "2016-06-24" @default.
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- W2361775969 date "2013-01-01" @default.
- W2361775969 modified "2023-09-25" @default.
- W2361775969 title "PROKARYOTIC EXPRESSION OF TROPOMYOSIN GENE FROM SOUTH CULTURED SEA CUCUMBER APOSTICHOPUS JAPONICUS" @default.
- W2361775969 hasPublicationYear "2013" @default.
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