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- W2361777186 abstract "To optimize the ISSR-PCR system for Blysmus sinocompressus,an orthogonal design was selected at four levels of five factors(Mg2+,Taq DNA polymerase,dNTP,primer and DNA template).Then,based on the optimal ISSR-PCR amplification system,ISSR primers with clear bands and the annealing temperatures were selected.As a result,the optimal PCR mixture(20μL)contained 1.80 mmol/L Mg2+,0.80 U Taq DNA polymerase,0.100mmol/L dNTP,0.6mol/L primer and 20 ng template DNA.Ten primers were screened with clear and consistent bands.With the optimal system of ISSR-PCR and primer screening,clear and steady bands were obtained in different individuals of Blysmus sinocompressus.The establishment of ISSR-PCR system could favor the genetic diversity of Blysmus sinocompressus pasture germplasm with molecular marker techniques.It was useful for the conservation of wild resource of Blysmus sinocompressus and excellent forage germplasm breeding." @default.
- W2361777186 created "2016-06-24" @default.
- W2361777186 creator A5044604763 @default.
- W2361777186 date "2014-01-01" @default.
- W2361777186 modified "2023-09-25" @default.
- W2361777186 title "Optimization of ISSR-PCR System and Primer Screening for Blysmus sinocompressus" @default.
- W2361777186 hasPublicationYear "2014" @default.
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