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- W2361792783 abstract "Objective: To investigate the biological and pharmacological characteristics of N-type calcium channel, and construct the different recombinants of subunits expressed in Xenopus oocytes. Methods; For expression studies, cDNAs encoding the α1B/α2δ /β1b subunits were transcribed to cRNA in vitro. α1B/α2δ/β1b, cRNA were injected to oocytes at a 1:1:1 concentration ratio respectively,and two-electrode voltage clamp was used to record the current. Results; The expressed N-type calcium channel currents in oocytes were fully blocked by ω-CTX MV Ⅱ A (1 FFUmol/L). α1B Expressed alone reached maximum current about 20 mV [(21.17±1.44) mV, (n = 7)]. Coexpression of the β1b with the α1B caused the shift of current-voltage relationship in a negative direction[ (12. 35±0. 88) mV, (n =7, P0.01)]. However, the current-voltage relationship was shifted in the positive direction by coexpression with α2δ subunit[(23.25 ± 1.25) mV, (n=7, P0. 05)]. Coexpression of α2δ/β1b/α1B produced the inward current in the hyperpolarizing direction [(17.08±1.86) mV, (n =7)]. Meanwhile, the voltage dependence of inactivation of recombinant N-type channel currents also was observed using the conventional double-pulse protocol. Half-inactivation potential was - 12. 0 mV for α1B alone, - 13.5 mV for α1B/α2δ, -51.4 mV for α1B/β1b, and -45. 9 mV for α1B/α2δ/β1b. Conclusion; The transient expres-sion system of N-type calcium channels in Xenopus oocytes was successfully established. The auxiliary subunits alone and in combination show different kinetic characteristics in the N-type channel, raising the possibility that subunit interaction contributes to the generation of functional diversity of N-type channels with wild-type neuronal preparations also, which provide the foundation for further study." @default.
- W2361792783 created "2016-06-24" @default.
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- W2361792783 date "2007-01-01" @default.
- W2361792783 modified "2023-09-25" @default.
- W2361792783 title "Electrophysiological characterization of different recombinant N-type calcium channel in Xenopus oocytes" @default.
- W2361792783 hasPublicationYear "2007" @default.
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