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- W2361800852 abstract "Objective To construct recombinant plasmid pLpmip and pcDNA3.1 mip of mip gene. Methods The mip gene was amplified by PCR from a template of Legionella pneumophila. The amplified DNA was ligated into pUC18 and pcDNA3.1(+) vector. The recombinant plasmid and pcDNA3.1 mip were identified by restriction analysis and PCR and further confirmed by sequence analysis. Results mip gene of 828bp was amplified. Recombinant plasmid pLpmip and pcDNA3.1 mip was constructed. Conclusion We amplified the mip gene of Legionella pneumophila, constructed recombinant plasmid pLpmip and pcDAN3.1 mip successfully." @default.
- W2361800852 created "2016-06-24" @default.
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- W2361800852 date "2004-01-01" @default.
- W2361800852 modified "2023-09-27" @default.
- W2361800852 title "Construction of the prokaryotic and eukaryotic recombinant plasmid of mip gene of legionella peneumophila" @default.
- W2361800852 hasPublicationYear "2004" @default.
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