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- W2361805927 abstract "To investigate the feasibility of using ~(19)F-NMR spectroscopy to monitor cyto-sine deaminase (CD) transgene expression in human colon cancer cell line. The SW1116cell line was transfected with a gene coding for CD from E. coli by the retroviral media-ted method. The assessment of the expression of CD gene in the colon cancer cell linewas carried out by RT-PCR, MTT assay of drug sensitivity to 5-FC and ~(19)F-NMR spec-troscopy. The results indicate that the transgenic SWCD, cell line was more sensitive tothe prodrug 5-fluorcytosine (5-FC) (IC_(50)= 66μmol/L) as compared to the parent cancercells SW1116 (IC_(50) = 16 mmol/L). After incubation of the cultured CD~+ cells in 774μmol/L 5-FC for 24 h, ~(19)F-NMR spectra of the intact cells showed two broad resonancescorresponding to 5-FC (taken up into cell chemical shift relative to trifluoroacetate of δ- 91. 7) and 5-FC produced by the action of CD (chemical shif of δ-93. 3), respective-ly. These results demonstrated the feasibility of using ~(19)F-NMR spectroscopy to nonin-vasively monitor CD gene expression in human colon cancer cells. This capability pro-vides a new approach for measuring gene expression that will be useful in clinical genetherapy trials." @default.
- W2361805927 created "2016-06-24" @default.
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- W2361805927 date "2004-01-01" @default.
- W2361805927 modified "2023-09-28" @default.
- W2361805927 title "ANALYSIS OF EXPRESSION OF CYTOSINE DEAMINASE GENE IN COLON CANCER CELLS WITH ~(19)F MAGNETIC RESONANCE SPECTROSCOPY" @default.
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