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- W2361839629 abstract "Objective To establish a rapid and sensitive multiplex PCR method for detection of methicillin resistant Staphylococcus and species specific identification of Staphylococcus aureus and Staphylococcus epidermidis. Methods A total of 80 S.aureus, 70 S.epidermidis and 60 the other coagulase negative staphylococci samples were used in this study. These isolates were obtained from Wuhan, China. Four pairs of primers targeting the mecA gene, femA gene, S.epidermidis species specific gene sequence, and the conserved sequence of bacterial 16srRNA gene were used in the multiplex PCR system under certain condition. Results In 210 Staphylococcus samples, the concordance of mecA gene detection and oxacillin resistance in the in vitro susceptibility test is 96.2%. The five isolates which were oxacillin susceptible but positive for mecA all showed their resistance after the selection of resistant cells under oxacillin concentration ranging from low to high. The three isolates, which were oxacillin resistant but negative for mecA were all susceptible to amoxicillin/clavulanate. So they may be the isolates with high production of β lactamase. Conclusions The multiplex PCR assay was used not only in diagnosis for the oxacillin resistance of Staphylococcus, but also in species specific identification for S.aureus and S.epidermidis. The method is highly efficient and specific." @default.
- W2361839629 created "2016-06-24" @default.
- W2361839629 creator A5056457613 @default.
- W2361839629 date "2004-01-01" @default.
- W2361839629 modified "2023-09-25" @default.
- W2361839629 title "Study of Multiplex Polymerase Chain Reaction for the Detection and Species-identification of Methicillin resistant Staphylococci" @default.
- W2361839629 hasPublicationYear "2004" @default.
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