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- W2361859994 abstract "Regard DNA of Myrica rubra as the template,several important parameters influencing RAPD of Myrica rubra were studied in order to establish the optimum RAPD system of Myrica rubra.The optimal RAPD-PCR system was established with orthogonal design.The results showed that a total volume of 20μl RAPD-PCR system consisted of 1×buffer,1.0u Taq DNA polymerase,3.0mmol/L MgCl_2,0.30mmol/L dNTPs,1.5μmol/L primer and 30-40ng template DNA.The suitable PCR procedure is one cycle denaturing at 94℃ for 3min,38 cycles which involves denaturing at 94℃ for 30s,annealing at 38℃ for 30s and extending at 72℃ for 90s,one cycle extending at 72℃ for 7min,and then kept at 4℃." @default.
- W2361859994 created "2016-06-24" @default.
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- W2361859994 date "2006-01-01" @default.
- W2361859994 modified "2023-09-23" @default.
- W2361859994 title "Study on Optimization of RAPD-PCR Reaction System of Myrica rubra using Orthogonal Design" @default.
- W2361859994 hasPublicationYear "2006" @default.
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