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- W2361862170 abstract "Objective To explore the effects of traditional Chinese medicine monomer Sal B on cytokine mRNA expression and activity of antioxidant enzymes of endothelial progenitor cells(EPCs) in vitro.Methods Cultivation and purification of EPCs in vitro was by density-gradient centrifugation and plastic adherence method,and identified by immunocytochemistry.EPCs were intervened by best concentration of Sal B,the expressions of VEGF,bFGF mRNA were detected by RTPCR,the activity of antioxidant enzymes SOD,GSH-Px activity of cell culture fluid were tested.Results VEGF,bFGF mRNA expression of Sal B group were increased,which had statistically significant difference compared with those of control group(P0.05).SOD activity was enhanced of Sal B group,which had statistically significant difference compared with that of control group(P0.05).Sal B group strengthened the trend of the activity of GSH-Px,but there was no significant difference compared with that of control group(P0.05).Conclusions Sal B plays a protective role and enhances its cell function on EPCs,the part of the mechanism is that enhances the expressions of VEGF,bFGF mRNA,promotes the cells to release SOD,GSH-Px,and enhances the scavenging capacity of free radical." @default.
- W2361862170 created "2016-06-24" @default.
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- W2361862170 date "2011-01-01" @default.
- W2361862170 modified "2023-09-25" @default.
- W2361862170 title "The influence of Sal B on cytokine mRNA expression and activity of antioxidant enzymes of the endothelial progenitor cells in vitro" @default.
- W2361862170 hasPublicationYear "2011" @default.
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