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- W2361865790 abstract "To investigate the feasibility of cryopreservation of bovine mammary tissue,bovine mammary tissue was cut into pieces with the diameter under 0.5 mm and frozen using cryoprotectant was DMEM/F12 including 20% NBS and 10% DMSO.The mammary tissue pieces were cryopreserved according to the following procedures: balanced at 4 ℃ for 1 h,-20 ℃ for 2 h,-40 ℃ for 2 h,and preservated at-80 ℃.After a week,recovered the mammary tissue and quantified the survival rate of tissue pieces.Cell viability was assessed on cell suspensions immediately after digestion and a 24 h culture period.The cells growth morphology was observed.It was found that recovery of bovine mammary tissue could adhere and grow,and the survival rate was 62.5%.After digesting,mammary cells could be isolated and the cell morphology from frozen-thawed samples was similar with fresh samples.The cells living rate was 58.5% when just isolated and it decreased to 46.3% after culture for 24 h.Long-term culture of mammary cells was carried out and a good growth status was offered.The mammary cells living rates of passages 1 increased to 93.2%.The frozen method of bovine mammary tissue was simple,convenient and fast,which could be used to maintaine mammary tissue viability,would stabilize and expand the source of mammary cells,and provide a stable material to study the structure and developmental biology of mammary gland." @default.
- W2361865790 created "2016-06-24" @default.
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- W2361865790 date "2011-01-01" @default.
- W2361865790 modified "2023-10-01" @default.
- W2361865790 title "Cryopreservation of Bovine Mammary Tissue and Isolation of Mammary Cells" @default.
- W2361865790 hasPublicationYear "2011" @default.
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