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- W2361902337 abstract "Objective:To construct expression system of Bombyx mori baculovirus expressing DC-SIGN protein,and to measure its biological activity.Methods:Human DC-SIGN cDNA was amplified by RT-PCR from the mature dendritic cells.The DC-SIGN gene was inserted into the baculovirus transfer vector pBacPAK8,and the recombinant plasmid pBacPAK8-DC-SIGN were cotransfected into Bombyx mori cell lines with the linearized Bm-BacPAK6 virus DNA.Then recombination in the cells and screening by the plaque assay,the recombinant virus Bm-BacPAK-DC-SIGN was obtained and again infected into Bombyx mori cell lines to express DC-SIGN protein which was detected by Western blot.The biological activity of the expressed DC-SIGN protein was measured by its combination with HIV-1 envelope glycoprotein(gp120).Results:Bombyx mori baculovirus expression system expressing human DC-SIGN protein was constructed,Human DC-SIGN protein was expressed and it can bind specifically with HIV-1 gp120.Conclusion:Human DC-SIGN protein was successfully expressed in expression system of Bombyx mori baculovirus,which showed the natural DC-SIGN protein-like biological activity.It found the basis of the antibody preparation against it and drug study on prevention and treatment of AIDS." @default.
- W2361902337 created "2016-06-24" @default.
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- W2361902337 date "2009-01-01" @default.
- W2361902337 modified "2023-09-27" @default.
- W2361902337 title "Study on DC-SIGN Protein Expression and Its Biological Activity Measurement" @default.
- W2361902337 hasPublicationYear "2009" @default.
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