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- W2361927573 abstract "By using the headspace collection and GC-MS, the differences in composition and concentrations of volatile allelochemics between transgenic Bt cotton and their parental varieties during various developmental stages were compared and analyzed. The cotton varieties DP5415-NUCOTN33 B, Simian3-GK2 and CCRI16-CCRI30 of both the parental variety and Bt transgenic cotton, were planted in an experimental field of Institute of Plant Protection, CAAS. The following plant parts in cotyledon-, three-leaf-, seven-leaf-, squaring-, flowering- and boll-stage, respectively, were used for volatile collections: cotyledon, top tender leaf, small square, floral organ, small boll. The samples were analyzed with GC-MS. HP6890 GC equipped with HP 5MS capillary column and HP5973 MS were used for chemical analysis. The carried gas was helium. The temperature program was as following: 40℃was maintained for 1 min and then the temperature was increased at 20℃/min to 100℃ and maintained for 10 min. The temperature at both the entrance and detector was 250℃. Current velocity of carried gas in GC was 50 ml/min and the split ratio was 28:1. The current velocity in column was 1.6ml/min. The source temperature of MS was 260℃. The main volatile compounds in cotton organs collected during various developmental stages were detectable and well separated with GC. The following compounds were identified: in cotton leaf— α-pinene, camphene, cyclohexene, β-pinene, β-myrcene, 1,3-pentadiene-3-methyl, β-hellandrene, D-limonene, 3-carene and a trace of hexen-2-ol-1; in cotton square and flower — α-pinene, β-pinene, β-phellandrene; in addition to the similar volatile allelochemics contained in square and flower, the cotton boll contained 3-carene as well. The dominant compound in cotton leaf was α-pinene (60%), and that in square, flower and boll was β-myrcene (80%). The results indicated that the components and ratios of main volatile allelochemics in various parts of cotton are different, thus it can be inferred that the chemical roles of these parts in interactions among plant, insect pests and natural enemies are different. The “t” test analysis indicated that the differences in main components and ratios of volatile allelochemics between transgenic Bt cotton and their parental varieties at different stages were negligible (all the t values t 0.05s), which suggested that the expression of Bt toxin protein in cotton had no effect on volatile profiles, and therefore there would be no potential effects on the interaction among plants, insect pests and natural enemies." @default.
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- W2361927573 date "2001-01-01" @default.
- W2361927573 modified "2023-09-24" @default.
- W2361927573 title "Analysis of volatile components in transgenic Bt cotton and their parental varieties" @default.
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